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NBI Super-Resolution Publications
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01 Cdk5-dependent phosphorylation of liprinα1 mediates neuronal activity-dependent synapse development
02 GAS2L1 Is a Centriole-Associated Protein Required for Centrosome Dynamics and Disjunction
03 A Distinct Pathway for Polar Exocytosis in Plant Cell Wall Formation
04 ATM protein is located on presynaptic vesicles and its deficit leads to failures in synaptic plasticity

Cdk5-dependent phosphorylation of liprinα1 mediates neuronal activity-dependent synapse development

PNAS Early Edition (2017).
Huiqian Huang1,2,3, Xiaochen Lin1,2,3, Zhuoyi Liang1,2,3, Teng Zhao4, Shengwang Du4, Michael M. T. Loy4, Kwok-On Lai1,2,3, Amy K. Y. Fu1,2,3,5, Nancy Y. Ip1,2,3,5

1 Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong, China

2 Molecular Neuroscience Center, The Hong Kong University of Science and Technology, Hong Kong, China

3 State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Hong Kong, China

4 Department of Physics, The Hong Kong University of Science and Technology, Hong Kong, China

5 Guangdong Provincial Key Laboratory of Brain Science, Disease and Drug Development, Hong Kong University of Science and Technology Shenzhen Research Institute, Shenzhen, 518057, China

The experience-dependent modulation of brain circuitry depends on dynamic changes in synaptic connections that are guided by neuronal activity. In particular, postsynaptic maturation requires changes in dendritic spine morphology...

GAS2L1 Is a Centriole-Associated Protein Required for Centrosome Dynamics and Disjunction

Developmental cell. 2017 Jan 9;40(1):81-94.
Franco K.C. Au1, Yue Jia1,Kai Jiang2, Ilya Grigoriev2, Bill K.T. Hau1, Yuehong Shen1, Shengwang Du3, Anna Akhmanova2, Robert Z. Qi4*

1 Division of Life Science and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

2 Cell Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands

3 Department of Physics and Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

4 Division of Life Science and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China. Electronic address: qirz@ust.hk

Mitotic spindle formation and chromosome segregation require timely separation of the two duplicated centrosomes, and this process is initiated in late G2 by centrosome disjunction. Here we report that GAS2L1, a microtubule- and actin-binding protein, associates with the proximal end of mature centrioles and participates in centriole dynamics and centrosome disjunction. GAS2L1 attaches microtubules and actin to centrosomes, and the loss of GAS2L1 inhibits centrosome disjunction in G2 and centrosome splitting induced by depletion of the centrosome linker rootletin. Conversely...

A Distinct Pathway for Polar Exocytosis in Plant Cell Wall Formation

Plant Physiol. 2016 Oct;172(2):1003-1018. Epub 2016 Aug 16.
H. Wang1,2, X. Zhuang1, X. Wang1, A.H.Y. Law1, T. Zhao3, S. Du3,4, M.M.T. Loy3, & L. Jiang1,5

1 School of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

2 College of Life Sciences, South China Agricultural University, Guangzhou 510642, China

3 Department of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

4 Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

5 CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China

Post-Golgi protein sorting and trafficking to the plasma membrane (PM) is generally believed to occur via the trans-Golgi network (TGN). In this study using Nicotiana tabacum pectin methylesterase (NtPPME1) as a marker, we have identified a TGN-independent polar exocytosis pathway that mediates cell wall formation during cell expansion and cytokinesis. Confocal immunofluorescence and immunogold electron microscopy studies demonstrated that Golgi-derived secretory vesicles (GDSVs) labeled by NtPPME1-GFP are distinct from those organelles belonging to the conventional post-Golgi exocytosis pathway. In addition...

A User-Friendly Two-color Super-resolution Localization Microscope

Opt Express. 2015. 23(2):1879-87.
Teng Zhao1, Ying Wang1, Yuanliang Zhai2, Xiaoxuan Qu2, Aifang Cheng2, Shengwang Du1 and M. M. T. Loy1*

1 Department of Physics, The Hong Kong University of Science and Technology, Hong Kong, China

2 Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong, China

We report a robust two-color method for super-resolution localization microscopy. Two-dye combination of Alexa647 and Alexa750 in an imaging buffer containing COT and using TCEP as switching regent provides matched and balanced switching characteristics for both dyes, allowing simultaneous capture of both on a single camera. Active sample locking stabilizes sample with 1nm accuracy during imaging. With over 4,000 photons emitted from both dyes, two-color superresolution images with high-quality were obtained in a wide range of samples including cell cultures, tissue sections and yeast cells.