FAQ

When do I need SR?

NBI SRiS is based on single molecule localization microscope, achieving 20nm in lateral direction, and 50nm in axial direction. In this scale, we can observe fine structure of cells, the distribution of proteins, protein-protein interaction and so on, which could not be accessed by conventional microscope.

As a newcomer of super-resolution imaging, how to quickly master the required skills and apply them to my own project?

In terms of instrument operation, our easy and seamless workflow can be picked up by a new user after ONE 3-hour training session. Although dSTORM is a new imaging technology, the sample preparation is in fact the general immunofluorescence staining procedures. The photoswitchable fluorescent dyes used for dSTORM, such as Alexa647 and Alexa750, are also widely used in conventional microscopy. Thus, it’s not difficult to kick off, and mastering sample preparation usually only requires some optimization of the fixation and staining conditions.

What is the format of the results? Can I use open-source software to analyse the data? Or what kind of data analyse tools can you provide?

The raw data can be saved as tiff files, and the localization data is saved in csv files, which can be analyzed using open source software such as Fiji. Our powerful analysis tools provide the users with ready-to-publish results. Furthermore, we also provide customized analysis tools for your data analysis.

How long does it take to capture one SR image?

It will take 5 to 10 minutes to get one SR image. It depends on factors such as the exposure time of each frame, the number of frame which in turn is determined by the structure complexity and the staining density of the targets.

How to prepare dSTORM sample?

In SRiS imaging, the sample is prepared by immunofluorescent staining. It should be noted that dSTORM requires photoswitchable fluorescent dyes (such as Alexa 647, Alexa 750) and adaptive imaging buffer. NBI will guide the customer to prepare the sample and buffer.

If my samples contain endogenous GFP or RFP fluorescent proteins, can I use them to do the super-resolution imaging? How to achieve?

Yes, GFP or RFP fluorescent proteins can be immunostained with GFP or RFP nanobody conjugated with photoswitchable dyes (such as Alexa 647, Alexa 750).

I always use confocal microscope, may I use the sample on NBI SRiS to perform super resolution imaging?

Yes, but it should be noted that for dSTORM, specific class of fluorescent dyes (Alexa 647, Alexa 750, etc) and imaging buffer with particular formula should be used. NBI will guide the customer to prepare the sample and buffer.

How can I ship the sample to your facility? Is it feasible if your facility is not local?

Customers who are close to our imaging service centers can send the samples to us in person and get the SR images. Other customers can send samples and reagents in an ice box to us by mail. For the details, please contact NBI staff.

Compared with other companies of similar products, what advantages does your company have?

1) Compared with similar commercial products, our NBI SRiS has special features such as simultaneous multi-channel imaging and active locking system, which makes it highly efficient and stable.[Learn More]
2) Besides the regular training and maintenance service, our biologists are ready to help the users optimize their experimental design for free. Benefiting from our imaging service to more than 100 researchers in the past, we are knowledgeable of different research areas and organisms.
3) We also provide customized hardware or software upon request. Contact us to get a quotation.

What kind of training do you provide?

If you purchase NBI SRiS, NBI provides a series of training, including hardware and software operation, sample preparation, imaging buffer preparation, data-analysis, manuscript drafting, etc.

We have got a microscope in our lab, can you help us upgrade it to super resolution? And how?

Our customized service includes system upgrading. Don’t hesitate to Contact Us to get a quotation.