Application

Examples & Scenarios.

Application areas & scenarios

Neuron Synapse

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Neuron Synapse

Super-resolution imaging of neuron synapse in mouse brain slices. (Fig. a, c & e) Widefield images of the synapse structures. (Fig. b, d & f) Super-resolution images showing the synapse and well-separated pre-synapse & post-synapse structures. (Images acquired with NBI SRiS 2.0, in cooperation with Herrup group in Hong Kong University of Science and Technology)

Mammalian cell organelles

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Golgi Apparatus

Super-resolution images of cis-Golgi network protein GM 130 and trans-Golgi network protein Golgin 97. (a) Widefield image shows the overlapping of these two proteins. (b) Super-resolution image clearly shows that the two proteins locate at two layers of golgi apparatus. (Images acquired with NBI SRiS 2.0, in cooperation with Guo Lab in Hong Kong University of Science and Technology)

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Mitochondria

3D Three-dimensional super-resolution images of mitochondria in COS7 cell. The mitochondrial outer membrane TOM20 was labelled indirectly. The mitochondria showed hollow tube structure. (Images acquired with NBI SRiS 2.0 by NBI)

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Organelles

Three-channel super-resolution images of COS7 cell organelles. (Fig. a) Mitochondrial outer membrane protein TOM20 (magenta), mitochondrial matrix protein COX IV (blue), and Golgi protein TGN46 (green). (Fig. b) Mitochondrial outer membrane TOM20 (red), nuclear membrane protein lamin (blue), and Golgi protein TGN46 were (green). (Images acquired with NBI SRiS 3.0 by NBI)

Yeast

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Yeast Organelle

Super-resolution images of tubulin proteins Tub1 and Tub4 in yeast spindle-pole body. GFP and RFP tagged tubulin proteins were transfected into the yeast cell, and further stained with GFP nanobody and RFP nanobody, respectively. (Fig. a & b) widefield images. (Fig. c & d) Corresponding super-resolution images, two-layer structure of γ-tubulin complex was resolved. (Images acquired with NBI SRiS 2.0, in cooperation with Dr Yuanliang Zhai in Hong Kong University of Science and Technology)

Bacterial

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Z-Ring Morphology

Three-dimensional super resolution imaging of the FtsZ Ring during cell division of the cyanobacterium Prochlorococcus. The six figures show the FtsZ ring assembly process during a cell division. The two movies show 3D FtsZ structure in two selected cells.

Chromatin organization

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Nucleic acid-FISH

Three-dimentional super-resolution images of telomere DNA. (Fig. a) Widefield image of single focus formed by telomere DNA. (Fig. b & c) Super-resolution images multiple foci, corresponding to the foci in the widefield image in the white boxes. Super resolution images show detailed structures, of which the changes with each focus could be easily identified. (Images acquired with NBI SRiS 2.0, in cooperation with Yong Zhao group from Sun Yat-sen University)